Evento

Introduction to Light Microscopy

Come learn how to use a microscope, an instrument to view the bewildering array of microstructures in our microworld with our friend Ken Klettke!

In this workshop you will learn the basics of light microscopy: how light sources are used and how optical elements create images, both real and virtual; the physics of light and physiology of eye; how lenses work and their types, and how to prepare your own slides using basic stains.

Stereo (dissecting) microscopes use reflected light to view larger 3 dimensional objects with reflected light, while traditional compound microscopes use transmitted light to view thin, smaller samples. Learn how the the four functional parts of a traditional compound microscope (light source, condenser, objective lens and ocular lens) contribute to the quality of the image, and how to setup up a microscope to optimize viewing (Köhler illumination).

Learn how to prepare samples using dry and wet mounts, and how different staining methods highlight specific aspects of a cell. Of the gazillion staining methods available, we will include hematoxylin & eosin (H&E) for tissue & cells, Gram stain for bacteria (both are used in medicine), and basic pen ink for onion cells (poor man's stain). Live creatures from fresh water and seawater from the Puget Sound will be viewed in specially prepared slides. We will also hunt for waterbears (tardigrades) in the moss & lichen outside the lab. Each participant will have their own microscope to use and the slides that you prepare are yours to keep.

Hope to see you there!