Evento

Introduction to Light Microscopy

Come learn how to use a microscope, an instrument to view the bewildering array of microstructures in our microworld.

In this workshop you will learn the basics of light microscopy: how light sources are used and how optical elements create images, both real and virtual; the physics of light and physiology of eye; how lenses work and their types, and how to prepare your own slides.

Stereo (dissecting) microscopes use reflected light to view larger 3 dimensional objects, while traditional compound microscopes use transmitted light to view thin, smaller samples. Learn how the the four functional parts of a traditional compound microscope (light source, condenser, objective and ocular lenses) contribute to the quality of the image, and how to setup up a microscope to optimize viewing (Köhler illumination).

Discover how to prepare samples using dry and wet mounts, as well as wax embedding in a home-made microtome, and how different staining methods highlight specific aspects of a cell. Of the gazillion staining methods available, we will include hematoxylin & eosin (H&E), Gram stain (both are used in medicine), and basic pen ink for onion cells (poor man's stain). Each participant will have their own microscope to use in the lab portion that follows the lecture part of the workshop. The slides that you prepare and the microtome you use are yours to keep.

Hope to see you there!